This detailed volume explores barley as both a crop and a model, with practical techniques such as crossing barley, a range of tissue culture methods, the preparation of barley tissues for different forms of microscopy, and the assessment of sensitivity to abiotic stresses. Efficient prools are provided for transformation, TILLING, virus-induced gene silencing and genome editing. There is also particular emphasis on a range of prools for genotyping and for the analysis of gene expression. Written for the highly successful
Methods in Molecular Biology
series, chapters include introductions on their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and easy-to-use,
Barley: Methods and Prools
serves as a valuable reference volume for cereal researchers and breeders by providing detailed prools covering important traditional skills such as crossing and tissue culture through to the latest technologies for genotyping, expression analysis, and genome editing.