Cell Imaging is rapidly evolving as new technologies and new imaging advances continue to be introduced. In the second edition of Cell Imaging Techniques: Methods and Prools expands upon the previous editions with current techniques that includes confocal microscopy, transmission electron microscopy, atomic force microscopy, and laser microdissection. With new chapters covering colocalization analysis of fluorescent probes, correlative light and electron microscopy, environmental scanning electron microscopy, light sheet microscopy, intravital microscopy, high throughput microscopy, and stereological techniques. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls Authoritative and cutting-edge, Cell Imaging Techniques: Methods and Prools, Second Edition is an easily accessible volume of prools to be used with a variety of imaging-based equipment likely available in a core imaging facility.