This new edition explores lab prools describing new techniques to study cytotoxic T-cells (CTLs), as well as chapters of a more general discursive nature, all with an emphasis on the use of systems biology in immunology. Beginning with phenotypical characterization of CTL populations, the volume continues with
in vitro
and
in vivo
cytotoxicity assays, methods to detect senescent T cells,
models to understand immune and bone cells cross-talk, microscopy and
imaging, as well as “Omics” approaches and molecular methods, concluding with chapters on CTL involvement in transplantation and link microbiota-immunity. Written for the highly successful
Methods in Molecular Biology
series, chapters feature the kind of detail and key implementation advice for best results in the lab.
Authoritative and up-to-date,
Cytotoxic T-Cells: Methods and Prools, Second Edition
serves as an ideal guidefor researchers working with these vital cells.