This book provides an in-depth exploration of various cytotoxic assays used in cell culture investigations, focusing on the development of therapies with insulin-releasing and insulin-like activities from food plant nutrients for controlling type 2diabetes. Key techniques and methodologies employed in this research are thoroughly discussed.Overall, the book presents promising insights into the use of food plant nutrients for developing alternative therapies for type 2diabetes, emphasizing the importance of further research to fully harness their potential.