As the highly anticipated update to
Lipid Second Messengers
(CRC Press, 1999),
Lipid-Mediating Signaling
is a current and comprehensive overview of research methods used in lipid-mediated signal transduction. Pioneering experts provide a much-needed distillation of a decade’s worth of advances in research techniques that are pertinent in understanding how lipid-mediated signal transduction ties to pathologic disorders.
Part I
describes methods used to identify activities of and assay the expression of different enzymes involved in lipid-mediated signaling.
Part II
highlights the use of mass spectrometry to ascertain the lipid content in various systems.
Part III
contains two chapters devoted to techniques used to determine the role of lipids in the activation of gene transcription.
Part IV
rounds out the book’s solid coverage by focusing on methods used to assess fatty acid uptake and metabolism.
is an unrivaled reference for this rapidly expanding, yet resource-limited field, and it is a valuable resource for for cell biologists, biochemists, and pharmacologists, as well as for researchers studying signaling transduction.