A collection of cutting-edge techniques for anyone interested in studying the molecular biology of mycobacteria, a medically significant organism responsible for both human and animal disease, including tuberculosis and leprosy. The book describes the basic techniques required for any study of mycobacteria, as well as providing methods for introducing DNA, for creating mycobacterial mutants, for obtaining defined gene knockouts by homologous recombination in both fast and slow growing species, for gene regulation and expression, for diagnostic purposes in detecting drug resistance, and for culture.