This volume aims at providing readers with prools to enhance their research for the next decade of phyhrome research. The chapters in this book cover topics such as tests for light-dependent interaction of phyhrome binding proteins using yeast two hybrid assays, in vitro pull down, and co-immunoprecipitation from stable transgenic lines; investigations into phosphorylation, SUMOylation, and ubiquitination of phyhromes and other proteins; a look at how in vivo spectroscopy can be used to measure Pfr levels of phyhrome
in planta
; identifying hormonal action and co-action in light-regulated processes; and ways to use the CRISPR/Cas9 system to generate
Physcomitrella
mutant lines. Written in the highly successful
Methods in Molecular Biology
series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive,
Phyhromes: Methods and Prools
serves as a guideline to researchers who are new to the field, and a stepping stone for experienced researchers looking to expand their knowledge of this evolving field.