This volume presents 30 state-of-the-art prools and reviews to set up and apply primary hepayte cultures for research and screening purposes. The first part of the book focuses on the use of these particular liver-based
in vitro
models to study the different aspects of the hepayte life cycle, including cell growth, differentiation and cell death. The second part of the book is targeted towards the demonstration of the applicability of primary hepayte cultures, or liver-based
models derived thereof, for functionality and toxicity testing. Written in the highly successful
Methods in Molecular Biology
series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory prools, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical,
Prools In In-Vitro Hepayte Research
is intended for basic and applied researchers in the area of pharmacology and toxicology, both in academic and industrial settings.