This second edition volume provides new and updated chapters detailing simple and accessible experiment prools to explore thymus biology. Chapters are divided into three parts presenting short reviews, analysis strategies, prools for cell preparation, flow cytometry analyses, Innate Lymphoid Cells (ILC), mouse T-cell development, antigen receptor-less cousins of T cells, bone marrow chimeras, thymic stroma, and multiple aspects of thymocyte biology. Written in the successful
Methods in Molecular Biology
series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible prools, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge,
T-Cell Development: Methods and Prools, Second Edition
aims to be a useful practical guide to help readers overcome obstacles associated with experimental approaches of T-cell development.