This detailed book contains chapters using unbiased approaches such as chemical and transposon mutagenesis, as well as a prool for allelic exchange, to make targeted mutants in
Staphylococcus
, as generating mutants of this bacteria can be accomplished in many ways, depending on the intended changes. Using these methods, the authors have been successful at making mutants that span individual single-nucleotide changes in the chromosome to whole genome mutant libraries. The latter strategy provides a useful tool for high-throughput screening, while single nucleotide changes are an elegant way of teasing apart the importance of single nucleotides in gene expression or to create specific amino acid substitutions to examine protein function. Written for the
Methods in Molecular Biology
series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.
Practical and authoritative,
The Genetic Manipulation of Staphylococci: Methods and Prools
serves as a vital resource and guide to scientists in the Staphylococcus community as they pursue their studies on these bacteria.