This volume mirrors the holistic feature of whole genome amplification (WGA) technology by combining reviews, detailed basic methods and advanced sample workflows. The first part of the book covers an overview of the development of WGA techniques throughout recent years including general considerations on bias in WGA, possible sample pre-enrichment strategies and how to run a single-cell lab. The second part focuses on major WGA methods and prools that allow the assessment of WGA product quality. The final chapters contain advanced prools and address issues such as sample preparation using laser-micro dissection; WGA from partially degraded DNA (formalin-fixed paraffin embedded samples); circulating tumor cells; and ancient samples. Written in the highly successful
Methods in Molecular Biology
series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools and tips on troubleshooting and avoiding known pitfalls.
Authoritative and thorough,
Whole Genome Amplification: Methods and Prools
will serve as a rich source of detailed information and inspiration and will help researchers, both new and experienced, yield successful results.