As predicted in the first edition, diseases can be predicted using the miRNA memory package (MMP) and miRNA entangling target sorting (METS), which could clarify the etiology of human diseases. Because researchers are investigating the relationship between circulating miRNAs and human diseases to develop new medicines, miRNA deep sequencing and detection methods will be high-speed and clever. is aimed at intermediate and advanced scientists, clinicians, students, and science enthusiasts. The Author hopes to lay the groundwork for future human health innovation.