A handy lab manual that permits quick and easy access to the techniques commonly used to analyse antibody specificity. Some of the most useful immunological techniques based on antibodies are described, including ELISA, immunoblotting and immunoprecipitation prools that provide useful tools for recognising immunological specificities, and basic immunofluorescence and immunohishemistry procedures for the in situ identification of antigens. The topics are discussed from a practical point of view, describing the theoretical basis of each technique and sample prools plus a troubleshooting guide. Attention is focused on the various aspects of the prools described thus providing the reader with the maximum possible information on each technique.