This detailed volume provides up-to-date guidance on techniques used to work with baculoviruses and insect cells. The book provides basic methods to create recombinant baculoviruses, to improve productivity, to produce a variety of products, to purify products, to quantify baculovirus sks or to quantify product produced, and it concludes with alternative uses of either baculovirus or insect cells as tools. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and comprehensive, Baculovirus: Methods and Prools serves as an ideal guide for researchers looking to overcome some of the limitations associated with the early baculoviral vectors and cell lines.