This book provides the fundamental aspects of bionanomaterials and bionanotechnology, and insight into the synthesis and modification of bionanomaterials in a detailed manner. It initiates with a general overview of biotechnology and nanotechnology followed by different strategies and methodologies for the synthesis of nanomaterials. Further, it discusses pertinent topics such as protein engineering, analysis, mechanisms of microbe- mediated nanosynthesis, followed by various challenges and innovation strategies, and the role of enzymes in bionanotechnology.