DNA may be everywhere and anywhere—in homes, at workplaces, during transport, and on personal items including clothing. DNA from a person may be on an object they have never contacted or in a room they have never entered. Concepts discussed in the book include non-self DNA on hands through day-to-day activities, the prevalence of background DNA in the environment and perhaps on the exhibit, the persistence of any DNA transferred, and that a DNA result will depend on these variables as well as recovery techniques. Since DNA may be transferred to an exhibit: (a) during the commission of a crime, (b) before the crime, and/or (c) after the crime through handling, examination, and testing, this book covers various transfer pathways and sources of DNA. Documented cases of inadvertent transfer of DNA resulting in wrongful convictions or the misdirection of criminal investigations are discussed—with an emphasis on contamination mitigation throughout the entire process. examines the additional complexity resulting from non-visible deposits of DNA that impact on sampling and testing regimes. The changing understanding of the composition of purported 'touch DNA' deposits from the skin, including extracellular DNA transported via body secretions, is described. Further, the newer focus on interpreting DNA evidence—using activity level propositions and the rationale and associated issues—is also discussed.