The term “systems thinking” has become synonymous with developing a coherent understanding of complex biological processes and phenomena. For researchers and educators alike, understanding how students’ systems thinking develops is an essential prerequisite to develop and maintain pedagogical scaffolding that facilitates students’ ability to fully understand the system’s complexity. To that end, this book provides researchers and teachers with key insights from the current research community onhow to support learners systems thinking in secondary and higher education. Each chapter in the book elaborates on different theoretical and methodological frameworks pertaining to complexity in biology education and a variety of biological topics are included from genetics, photosynthesis, and the carbon cycle to ecology and climate change. Specific attention is paid to design elements of computer-based learning environments to understand complexity in biology education.