This volume explores the latest ways to detect hypoxia in the context of cancer, including techniques to study gene expression changes, protein responses, and cellular adaptations to low-oxygen conditions. This book also covers new prools to characterize hypoxia in tumors with high spatial resolution and novel therapeutic approaches that consider the complex microenvironments of solid tumors. Written in the highly successful series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, is a valuable tool to help cancer researchers and other scientists detect hypoxia in cancer and other disease pathologies.