This third edition is comprised of well-established prools that are considered the gold standard in the field as well as new methodologies for mihondrial DNA analysis. of
Mihondrial DNA: Methods and Prools
describes prools for detecting mutations in mihondrial DNA, techniques to assess mihondrial DNA damage, visualization of mihondrial DNA
in situ
, detection of mihondrial DNA nucleoids within the mihondria, methods for analyzing mihondrial DNA replication, mihondrial DNA-encoded protein translation and mihondrial DNA copy number, the latest technologies for modifying the mihondrial genome and methods for the purification of proteins involved in the replication and transcription of mihondrial DNA. Written for the
Methods in Molecular Biology
series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical,
Mihondrial DNA: Methods and Prools, Third Edition
will be useful not only for mihondrial researchers but also for scientists studying human diseases where mihondrial DNA variation has been recognized as an important pathogenic factor, including cancer and neurodegeneration.