Results indicate that the hiking trail negatively influences species distribution but have minimal effects on plant assemblages. The concept of Biodiversity Net Gain (BNG) is essential to mitigate these impacts by enhancing degraded habitats, promoting sustainable trail management, and ensuring that conservation actions offset ecological disturbances. Implementing BNG principles through habitat restoration, buffer zones, and sustainable tourism practices will improve biodiversity outcomes in SKP. Based on the findings, relocating the hiking trail to the slopes of Mount Sinai away from the basins (Farshs) is advisable to mitigate biodiversity impacts.