This second edition provides update and new chapters detailing core and emerging in vitro and in vivo prools. Chapters guide readers through cellular and molecular biology approaches, in vivo genetic approaches, various “omics”-based strategies, therapeutic strategies, and advanced techniques in the fields of tissue engineering and nanotechnology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, The Tumor Microenvironment: Methods and Prools, Second Edition is a valuable resource for both novice and expert scientists in this developing field.