The Gene analysis of Diabetes Mellitus will cover the basic aspect of the Type 2 Diabetes Mellitus T2DM comparing with prediabetic and Control, here the aim is to focus on to show the identifies and recognised Single nucleotide Polymorphism (SNPs) and pseudogenes analysed in Diabetic. Prediabetic individuals compared with control (Healthy). In this book well, good aspect of Gene Ontology has also been covered where Cellular components, Biological Process and molecular functions of each gene has been documented, correlating with the metabolic pathways regulating the energy flow of Diabetic, prediabetic and Healthy Subject.