This book constitutes the refereed proceedings of the 16th International Conference on Artificial General Intelligence, AGI 2023, held in Skholm, Sweden in June 2023. The 35 full papers and one short paper presented in this book were carefully reviewed and selected from 72 submissions. The papers cover topics from foundations of AGI, to AGI approaches and AGI ethics, to the roles of systems biology, goal generation, and learning systems, and so much more.