This volume explores the evolution of bacterial cancer therapy and describes the modern techniques used in therapy today. The chapters in this book cover a broad range of topics such as the development of tumor-targeting A1-R, a microfluidic device for precise quantification of the interactions between tumor-targeting bacteria and tumor tissue, non-invasive in vivo imaging of bacteria-mediated cancer therapy using bio-luminescent bacteria, methods to achieve remote-control of therapeutic gene expression in tumor-targeting bacteria, and cell-cycle decoy of cancer cells resistant to cytotoxic drugs to drug sensitivity by A1-R. This book concludes with a chapter on the future potential of bacterial therapy of cancer. Written in the highly successful series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls. Thorough and informative, is a valuable resource for anyone who is interested in cancer and bacterial therapy.