The reader will learn the importance of physiological cell, tissue and organ models and gains important knowledge on 3D analytics. This textbook deepens selected aspects of the textbook “Cell Culture Technology”, which also is published in this series, while offering extended insight into 3D cell culture. The concept of the textbook encompasses various lectures ranging from basics in cell cultivation, tissue engineering, biomaterials and biocompatibility, test systems and regenerative medicine. The textbook addresses Master- and PhD students interested and/or working in the field of modern cell culture applications and will support the understanding of theessential strategies in 3D cell culture and waken awareness for the potentials and challenges of this application.