This volume provides updated technical approaches that have been developed to characterize monoterpene indole alkaloid metabolism in from metabolite/gene product localization, alkaloid chemical synthesis, candidate gene prediction, transcription factor characterization up to functional genomic tools based on gene overexpression. Written in the format of the highly successful series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible prools. Authoritative and cutting-edge, aims to be a guidebook to all researchers working at characterizing alkaloid biosynthesis and more broadly specialized metabolisms