This second edition of updates several techniques from the first edition together with novel and -based methodologies. Written by internationally recognized expert authorities, this volume provides vital techniques from eosinophil purification to experimental modelling, with each technique spelled out in clear and straightforward terms, assuming no previous knowledge of the method and without necessitating sourcing additional information elsewhere. Written in the highly successful format, chapters include introductions to their respective topics, lists of the essential materials and reagents, step-by-step, readily reproducible laboratory prools, with tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, provides the practical means to extend our knowledge of eosinophil function in health and disease, underpinning research that may in turn lead to new hypotheses for future examinations into the role of this intriguing and enigmatic leukocyte. Chapters 10 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.