Reflecting the expanding field’s need for reliable prools, offers techniques from a worldwide team of experts on this versatile and vital subject. The topics covered fall into four broad categories: steady-state fluorescence spectroscopy, time-resolved fluorescence spectroscopy, fluorescent probe development, and the various sub-categories of fluorescence microscopy, such as fluorescence recovery after photobleaching (FRAP), live cell FRET imaging (FRETim), fluorescence lifetime imaging (FLIM), fluorescence fluctuation spectroscopy (FFS), and single-molecule fluorescence spectroscopy (smFS). Written as a part of the popular series, chapters include the kind of unambiguous detail and key implementation advice that proves essential for successful results. Comprehensive and practical, aims to guide both ‘novice’ and established scientists toward furthering their research with these invaluable techniques.