This book covers the following topics: Recombinant DNA technology Restriction endonucleases T4 DNA ligase Plasmid cloning vectors pBR322 plasmid pUC19 plasmid Bacteriophage λ vectors Cosmids Transformation and selection Cloning DNA sequences that encode eukaryotic protein Synthesis of cDNA Creating and Screening a library Library construction methods Random mutagenesis methods Recombination-based methods Homology-dependent methods Homology-independent methods Targeted mutagenesis methods Selection/screening for desired property Genotype-phenotype linkage DNA sequencing techniques PCR Protein engineering Directed evolution Applications of directed evolution tool Rational design and site directed mutagenesis