The second edition of captures all the new exciting developments in the field of microfluidics over the last twenty years. While maintaining the same clear structure, and accessible explanations of the basic theory, this new edition is a complete revision of the first edition and makes use of the considerable data collected in the field over the last two decades. The book describes the applications, the market, and attempts to envision the future of microfluidics. It covers the physics of miniaturization, the hydrodynamics of microfluidics in channels and with droplets, transport phenomena in microsystems, electrokinetic phenomena, and an introduction to microfabrication. The basic principles are explored in depth and with rigor, and their main applications are clearly presented. Many examples are provided and discussed simply, most often from a physical perspective, and the book includes 415 figures and 600 references. Offering a cross-disciplinary view of the field embracing biological, chemical, physical and engineering perspectives, this book is an ideal resource for students and researchers at any level.