This practical manual on molecular diagnostics is written to help the students and faculties who are doing practical of molecular biology, biochemistry and biotechnology. This practical manual covers the syllabus of ICAR as per 5th dean committee for B.Sc. This practical manual can also be used by the students of under graduate and post graduate of other streams of biological sciences. This manual explains step wise laboratory practical procedure for different experiments. This manual will also be helpful for those students and researchers who want to plan the experiment in molecular biology laboratory. The authors are also engaged in research and teaching in this area and having wide experience in molecular biology. In the end of each experiment some questions have been asked to solve by the students that will help in better understanding of the topic. This manual also contains set of objective questions related to the molecular diagnostics that will be very useful for their competitive exams and regular theoretical exams as well.