The book includes an introduction about fundamental information of sterilization process, protocols related to plant tissue culture and molecular biology which deals with principle, reagent preparation details, procedure and precautions of about the same. Necessary Knowledge of Plant Tissue Culture and Molecular Biology has been given in effortless and easy to understand language. Plant Tissue Culture exercises such as Media preparation, Sterilization technique for explants protocol, Cell Suspension culture, Shoot Tip Culture, embryos Culture, protoplast isolation, and haploid plant production; Molecular Biology exercises such as plasmid DNA isolation, DNA Isolation from various sources, organelles DNA isolation, rna Isolation, Digestion, Ligation Agarose gel electrophoresis and its elution, various cloning methods have given in very simple student friendly manner.