With its complex and extensively regulated metabolism, the study of the RNA lifecycle demands tools that allow for the localization of RNAs to be observed either in an in situ setting or, preferably, under in vivo conditions. In , the best and brightest investigators provide an up-to-date and in-depth description of basic methods and prools used for detecting and visualizing mRNAs in both fixed and live cells, from bacteria to mammals. For novices and experts alike, this mix of classic in situ hybridization and advanced live imaging techniques, cell fractionation and affinity purification procedures, and bioinformatics tools gives researchers the most complete and extensive array of research aids possible. As a volume written in the highly successful series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and expert tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, offers well-honed techniques in order to inspire researchers around the world to further our knowledge of the vital biological significance of RNA.