This volume provides a comprehensive collection of current methods and prools to study posttranscriptional base modifications in RNA with special focus on methylation. The prools in this book discuss state-of-the-art methods for investigating aspects of RNA methylation on different types of RNA. The prools cover topics such as wet-lab techniques for the detection of methylation, instructions for bioinformatics analyses of transcriptome-scale data, and prools for the functional examination of RNA modifications and enzymes. Written in the highly successful series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, is a valuable resource for biochemists and molecular biologists, from various fields, who wish to investigate different types of RNA methylations.