Facing new challenges with respect to sustainable development strategies for low-income countries, related to global environmental change and globalization of markets, an interdisciplinary Wageningen research group set out to draw lessons from the DLO-IC projects of the last eight years, stimulate discussion and support identification of a future research agenda for RDSA. In that process, special emphasis was given to lessons for future thinking about the contribution of agriculture to: 1) poverty alleviation and rural livelihoods; 2) food security, human nutrition and health; 3) environmental sustainability and natural resource management. In discussing the way ahead, a number of major research challenges, as well as policy questions are outlined. These include the interrelations between climate change and food security and environmental degradation, increased demand for biofuels and animal feed, and competing claims on natural resources, and rural development and livelihood strategies in low-income countries.