This updated and expanded volume reflects the current state of the structural protein field with improved and refined prools that have been applied to particularly challenging proteins. Beginning with a section on structural bioinformatics, the book continues with sections covering the challenge of producing high quality samples for structural studies, particularly mammalian membrane proteins and protein complexes, as well as prools for structure determination, including the use of electrons in structural biology and more. Written for the highly successful series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, will aid researchers in expanding our knowledge of this vital and expansive area of protein science. Chapter 9 is available open access under a CC BY 4.0 license.