With this English edition of , Serena Di Nepi traces the troubled and compelling history of the birth of the ghetto in sixteenth-century Rome. From the arrival of the Sephardim to the Italian wars, and the incredible story of an accusation of ritual homicide that was never made, the research sketches a picture of Jewish society, its institutions and its ruling class during the first fifty years of segregation. How did Jews react to the ghetto? Did their institutional organization change, and how? What was the impact of the restrictive laws regarding their professions and their working environment? What was the role of the rabbis in such a problematic moment? What became of Rome’s Jewish bankers? This book addresses these questions.