This volume is timely and looks into the updated aspects of mutagenesis, TILLING, Eco-TILLING along with OMIC tools, their amalgamated applications towards crop improvement. This book contains 11 chapters and 250 pages authored by globally reputed scientists on the field of mutagenesis, TILLING and Eco-TILLING. This book is useful for research scholars, students, teachers and scientists in the academia, policy makers, relevant public, plant breeding companies, private companies and cooperatives interested in understanding or applying mutagenesis, TILLING for editing gene of interest and develop new products in agriculture.