Solanum melongena L in a vegetable crop widely cultivated in Mediterranean area. Egg plant is widely used as medicinal plant in addition to beginning an important vegetable in many dietes. Some medicinal uses of egg plant tissues and extracts, includes, treatment of diabetes, asthma, cholera, bronchitis and dyheria. Its leaves and fruits are reported to promote lowered blood cholesterol levels. Roots are credited in the indigenous medicine as anti asthmatic and general stimulant. Considering the importance of the species Solanum melongena L an attempt has been made to achieve the some objectives using the tissue culture technology for improvement of the species. To establish the protocol for callusing efficiency of cotyledon and leaf explants on medium containing various growth regulators. To standardize the protocol for callus mediated regeneration on different concentrations and combinations of growth regulators. To study the effect of various concentrations and combinations of growth substances on in vitro plantlet regeneration through direct organogenesis in leaf and cotyledon explants. To standardize the protocol for Agro bacterium mediated genetic transformation from hypocoty